Journal: American Journal of Translational Research
Article Title: Circ_0039569 promotes renal cell carcinoma growth and metastasis by regulating miR-34a-5p/CCL22
doi:
Figure Lengend Snippet: CircRNA expression profile in RCC tissues. A. Heat map showed the significantly expressed circRNAs with 5 folds were presented. In the heat map, red color: the upregulated circRNAs with high fold changes; green color: the downregulated circRNAs with high fold changes. B. Scatter plots are used to evaluate the difference in the expression of circRNAs between experiment group and control group. The values plotted on X and Y axes are the averaged normalized signal values of each group (log2 scaled). The middle green line refers to no difference between the two groups. And the flank green lines represent 2.0 fold changes. The circRNAs above the top green line and below the bottom green line indicate more than 2.0 fold changes between two groups. C. Six circRNAs including circ_0036840, circ_0082616, circ_0017724, circ_0039569, circ_0039569, and circ_0062705 were chosen for validation in the RCC tissues using qRT-PCR. D. Six circRNAs including circ_0086458, circ_0019692, circ_0037923, circ_0040865, circ_0051398, circ_0039151 were chosen for validation in the RCC tissues using qRT-PCR. Data were expressed as mean ± SD. **P < represents statistical difference.
Article Snippet: Finally, these labeled RNAs were hybridized using Arraystar mouse circRNA Array (V1.0, Arraystar), and scanned by the Agilent Scanner G2505C.
Techniques: Expressing, Quantitative RT-PCR